| Research findings of - S.I.V.A drops |
Membrane bound adhesion of S.I.V.A drops molecules in the peritoneal phagocytes both during in vitro and in vivo tests was observed.
To reconfirm the boosting effect of SI.V.A on macrophage, we have conducted the challenge study, using candida as pathogen. the phagocyte mediated immunity was impaired with immuno suppressive drug and was challenged with candida cells .when S.I.V.A was administered ,the diseases per se was very insignificant when compared to S.I.V.A non treated control. This findings proves that S.I.V.A offers a very protective immuno boosting .
An effective immune surveillance and timely defence of the host system by the phagocytes (macrophages) are inventible for disease free life from a variety of pathogenic and opportunistic microbes that encompass us in the environment. S.I.V.A, the herbal formulation selectively boosts the phagocytic ability of the macrophages located at different regions of the body such as peritoneal cavity, alveolar space etc., |
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S.I.V.A, a poly herbal proprietary oral preparation for boosting the macrophage (phagocytes) mediated immunity both by in vitro and in vivo carbon exclusion technique. |
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Specific effect of S.I.V.A treated phagocytes in killing of Candida cells was also established by uptake of tryphan blue staining method . |
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| The present study clearly demonstrates that, S.I.V.A offer Sufficient immune protection especially in the disease per se . Of experimental candidiasis (Candida albicans ) . Tryphan blue staining (live cell staining) also revealed that death ratio of the fungal cells (Candida albicans and Aspergillus niger spores) ingested phagocytes in the test group (S.I.V.A treated phagocytes) was in the range of 20-30 % as against 60-70 % death in the case of control group . |
| Research Study : |
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Effect of S.I.V.A on isolated macrophages in tissue culture medium such as RPMI 1640 supplemented with TC199 in the laboratory revealed that, the phagocytic ability of the treated cells being increased to 40 folds when compared to the un treated cells. |
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Tryphan blue staining ( live cell staining) also revealed that death ratio of the fungal cells (Candida albicans and Aspergillus niger spores) ingested phagocytes in the test group (S.I.V.A treated phagocytes) was in the range of 20-30 % as against 60-70 % death in the case of control group |
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| Group |
No. of phagocytes
/ ingested spores |
Phagocytic index
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| S.I.V.A treated cells |
10 / 192 |
1:19 |
| Untreated control |
10 / 82 |
1:8.2 |
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In vivo confirmation :
Immuno boosting ability of the phagocytes was further confirmed in Rabbit model using carbon exclusion technique. This study further validates the above fact that carbon exclusion occurs more effectively in the S.I.V.A treated animals than the control.
Mechanism of action :
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Increased membrane stability and chemotacticity of the phagocytes while treatment with S.I.V.A is suspected. |
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Membrane bound adhesion of S.I.V.A molecules in the peritoneal phagocytes both during in vitro and in vivo tests were observed |
Pharmacokinetics :
Onset of immune boosting activity occur in 4-5 days of oral administration of 30 mg of S.I.V.A drops.
Pharmacokinetic parameters after 30 mg of single oral dose of SIVA (14 days after administration)
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| Pharmacokinetic parameters |
Plasma / body fluids |
| AUC (0-24 h) ng / ml |
1427, 824,1101 (3 peaks) |
| Cmax ( ng / ml) |
138.8 |
| Tmax (h) |
6.4 |
| Cmin |
14.2 |
| Terminal half life (h) |
18.0 |
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| Enhanced Phagocytosis by S.I.V.A |
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Time to peak concentration (Tmax) :
Seven major peaks were recorded after oral administration of 30 mg of S.I.V..A single dose in animals after 1 hr.
Duration of activity :
Six to seven hours after administration, 1 of the 7 peaks could be recorded. Due to the poly-herbal nature of the drug, the mean plasma concentration could not be established.
Metabolism :
Extensive in liver and spleen.
Excretion :
Elimination of S.I.V.A is not clearly known. Levels could not be detected in urine and faeces. Single peak of the 7 peaks could be detected in renal cells after 17.3 hrs
Effect of honey :
Extent of absorption is enhanced significantly when honey is co-administered. Enzyme based activation is presumed for the enhanced activity.
Drug interaction :
Drug interaction of S.I.V.A with other similar line of drugs or with any other allopathic drugs was not observed.
Penicillin group of antibiotic efficacy is retarded during S.I.V.A administration
The process of Phagocytosis of an antigen (bacteria) |
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| Phagocytosis of an antigen by untreated and S.I.V.A treated phagocytes |
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