Anti
allergenic study of G - 7 Capsule |
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G-7
capsule, the proprietary formulation of Dr.JRK’s
Siddha Research and Pharmaceuticals Pvt.Ltd.,
Kuntrathur, Chennai was studied. |
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Both
In vitro and In vivo study was carried out for confirmation.
The peritoneal mast cells were used for the in vitro
study. The isolated mast cells were maintained in RPMI
1640 tissue culture medium in a flat bottom multi welled
micro titre plate. The cells were treated with different
concentrations of G-7 extract prepared in Hans buffer
with the aid of Myosol as solvent. The concentrations
of the G-7 tested were 2,10,20,40 and 50 µg /
ml of medium. The treatment time was kept constant for
20 minutes. Later, the cells were challenged with sensitized
IgE and the opsonized allergen. The amount of histamines
and neutral proteases released by the G-7 treated and
untreated mast cells were quantified. Histamine quantification
was done by radio immune assay using radio tagged antihistamines
using Scintillation counter. Similarly, attempts were
made to check the histamine release by using specific
immune staining. |
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Treatment |
Quantity
of Histamine released |
Protease
activity |
Treated
Group 40µg / ml |
60
IU / 100 cells |
18.3
mg / ml / minute |
Untreated
Group |
123
IU / 100 cells |
28
mg / ml / minute |
|
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Proteolytic
activity was determined by substrate-enzyme reaction
assay. The end product the tyrosine released as a result
of proteolyses of collagen is determined. |
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 Determination
of mechanism of action |
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The
IgE – FC receptor site in the mast cells were
blocked with a dummy protein prior to G-7 treatment.
The dummy protein is later removed and was challenged
with sensitized IgE. The findings revealed that the
degranulation inhibition is mediated through IgE receptor
binding by the G-7 moiety. |
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| In
vivo study |
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| Dust
inhalation study |
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For
in vivo confirmation of the effect of G-7 in
mast cell degranulation inhibition, dust inhalation
study was conducted using Rabbit model. Rabbits were
pre-treated with G-7 for 15 days. Later the treated
and untreated animals were challenged with house dust
through a nebulizer in a dust inhalation chamber. The
histamine released in the system is assayed as mentioned
above. |
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The
findings of the study reveal that G-7 offers greater
protective effect to the mast cells from degranulation
during the allergen exposure |
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Inhibition
of broncho spasm during allergen exposure and anaphylactic
shock in the case of - G
-7 treated animals also observed in a separate study. |
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| Mechanism
of action of G - 7 on mast cells |
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|
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Research
findings of -
A - FORTE |
|
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| A-Forte
Capsule is the Proprietary work of Dr.JRK's Siddha
Research & Pharmaceuticals (P) Limited. |
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Studies
on normal adults have shown that A - Forte capsule
increases the mean level of R.B.C.,
vital capacity and total protein. It causes significant
rise in hemoglobin content, with simultaneous
decrease in mean blood urea and serum acid phosphate. |
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A-forte contains Withania somnifera.
The plant’s therapeutic properties have
been extensively studied and documented. The alcoholic
extracts have significant anti-inflammatory
properties and it was confirmed on studies adrenoectomised
rats. Ethanol extracts have shown anti-spasmodic
and along with the alkaloid fraction showed hypotension.
Acetone soluble fractions and their sub-fractions
( alkaloids) exhibited muscle relaxant and anti-spasmodic
effect besides potentiation of hexobarbital sleeping
time. |
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Anti-stress ( adaprogenic) activity was
assessed in experimental models of stress. The
drug increased physiological endurance,
protected animals from stress-ulcers and CCl4
induced hepatotoxicity . The use of W. somnifera
finds extensive references in texts of Ayurveda
and Siddha as a tonic and vitalizer
and the clinical use for prevention and treatment
of stress during ailments like psoriasis, vitiligo,
artereo sclerosis, premature ageing, arthritis,
diabetes, hypertension and malignancy. It can
also act as an immunomodulator. Its aphrodisac
and invigorative properties are
also well researched |
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A - Forte
also contains Centella asiatica
. The plant’s therapeutic properties have
been extensively studied and documented. Many
active compounds have been isolated from the extracts
of the plant parts. Asiaticoside- an isolate from
the plant, showed anti-tubercular activity and
also inhibited the growth of mideprae. Ethanol
extracts showed anti-spasmodic
activity. Both alcoholic and aqueous extracts
of C. asiatica exhibited relaxation of isolated
pleur of rat and antagonized BaCl2 induced spasm
and reduced spontaneous contraction. |
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The
herb when administered for a period of 12 weeks
produced behavioural improvement
in mentally retarded children and the consistency
of I.Q. the alcoholic extracts produced tranquilizing
effect in rats. The drug was tested on
43 normal adults and was found to increase
the mean level of RBC blood sugar, serum
cholesterol, vital capacity and total proteins.
The drug also caused significant improvement
of Hemoglobin content with simultaneous
decrease in mean blood urea and serum acid phosphate. |
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